DOI Prefix : 10.9780 | Journal DOI : 10.9780/22307850
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Volume : III, Issue : XI, December - 2013

Visualization Of n Accumulation In Two Cultivars Of Barley Plants Using A Positron-emitting Tracer Imaging System (petis)

Salwa Abdel-Latif , Hanan M. Abou-Zeid

DOI : 10.9780/22307850, Published By : Laxmi Book Publication

Abstract :

The translocation of nitrate in 10-day-old intact plant of barley (Hordeum vulgare L.) Steptoe (wild type) and Az12(mutant), starting from soaking was visualized using the positron-emitting tracer (PETIS), 13Nlabelled nitrate (half life of 9.96 min) was supplied to N-deficient and N-sufficient barley seedlings at 9-day-old seedling. Nitrate-deficient barley seedlings showed negligible accumulation of short-lived tracer 13NO3- in shoots than did N-sufficient barley and in Az12 (mutant) more than in Steptoe (wild type) genotypes revealing that the Nsufficient seedlings caused enhancement of nitrate uptake and translocation to shoots. The mechanism of accumulation will be discussed.

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Cite This Article :

Salwa Abdel-Latif , Hanan M. Abou-Zeid, (2013). Visualization Of n Accumulation In Two Cultivars Of Barley Plants Using A Positron-emitting Tracer Imaging System (petis). Indian Streams Research Journal, Vol. III, Issue. XI, DOI : 10.9780/22307850,

References :

  1. 1.Abdel-Latif, S.; Kawachi T.; Fujikake H.; Ohtake N.; Ohyama T.; Sueyoshi K. 2004. Contribution of shoots and roots to in vivo nitrate reduction in NADH-specific nitrate reductase deficient mutant seedlings of barley (Hordeum vulgare L.). Soil Sci. Plant Nutr., 50: 527–535.
  2. 2.Aslam, M.; Travis R.L. and Huffaker R.C. 1992. Comparative kinetics and reciprocal inhibition of nitrate and nitrite uptake in roots of uninduced and induced barley (Hordeum vulgare L.) seedlings. Plant Physiology, 99: 1124–1133.

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