DOI Prefix : 10.9780 | Journal DOI : 10.9780/22307850
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Volume : III, Issue : XI, December - 2013

Effect Of Gelling Agent And Salt Strength Of Nutrient Medium On INVITRO Growth Of MOMORDICA CHARANTIA

Hassanien S.H., Ibrahim I.A., Emara H.A., Bekhit M.H., Enass S. Amer

DOI : 10.9780/22307850, Published By : Laxmi Book Publication

Abstract :

This investigation aimed to study the effect of two types of gelling agent (agar and gelrite) on in vitro growth of Momordicacharantia. Agar was tested at the concentrations (5g/l, 7g/lor 10g/l), while 1.5g/l, 2.5g/lor 3.5g/lgelritewere used.Shoot tip and nodal cutting were used as two types of explant. Nodal cutting explants showed the best performance for in vitro growth. Data indicated that,7g/lagar gave the highest values of callus formation percentage and both of fresh and dry weight of callus, while gelrite was effective in increasing the shoot and root formation, as 2.5g/l gelrite significantly increasedthe percentage of shoot and root formation. However, both number of shoots and nods showed to be highest when 3.5g/l gelrite was added to the medium.Shoot length was significantly highest with 1.5g/l gelrite. Data clearly indicated that, highest percentage of survived plantlets which transferred to pots was obtained with 7g/l agar, but 1.5g/lgelrite gave no survival results. Hyperhydricity was significantly increased when 1.5g/l gelritewas used with nodal cutting explants whichexcised from plantlets grown on medium contained 2 mg/l BA,thensub-cultured on free- growth regulators MS medium. Data were detectedafter 4 weeks of incubation. For detection the effect of MS nutrient salt strength on in vitro growth using 7g/l agar or 2.5g/l gelrite with nodal cutting explant,data showed that,half strength of MS medium contained7g/l agar significantly increased callus formation percentage.While full strength MS medium contained2.5g/l gelrite significantly increased the percentage of shoot and root.Different MS nutrient strengths (0.5, 1.25 or 1.50) gave highest number of shoots without significant difference; however they significantly surpassed full strength of MS in that, concern. Moreover the highestnumber of nods was observed with 1.5 strength of Msnutrient mediumcontained 2.5g/l gelrite. The highest value of shoot length was recorded by using 1.25 strength of MS nutrient medium contained 2.5g/l gelrite.

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Hassanien S.H., Ibrahim I.A., Emara H.A., Bekhit M.H., Enass S. Amer, (2013). Effect Of Gelling Agent And Salt Strength Of Nutrient Medium On INVITRO Growth Of MOMORDICA CHARANTIA. Indian Streams Research Journal, Vol. III, Issue. XI, DOI : 10.9780/22307850,

References :

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