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Volume : III, Issue : XI, December - 2013 Effect Of Pretreatment And Media Composition On IN VITRO Propagation Of MOMORDIA CHARANTIA Using Different Explants. Hassanien S.H., Ibrahim I.A., Emara H.A., Bekhit M.H., Enass S. Amer Published By : Laxmi Book Publication Abstract : This investigation was conducted to study the effect of dark pretreatment with different thermal conditions (32±2 ○C or 22±2 ○C), explant type and growth regulator concentrationson callus production and regeneration of Momordicacharantia. Explants cultured on MS medium contained different concentrations of plant growth regulators with Gamborg's vitamins.All explants that were exposed to dark at 32±2 ○C showedthe highest callus percentage compared to the cultures exposed to dark at 22±2 ○C.MS medium contained 0.5 mg/l TDZ + 1.5 mg/l NAA with Gamborg's vitamins showed to be the effective medium for callus induction. Data indicated that both cotyledons and hypocotyls gave the highest percentage of callus formation after one week of incubation at dark. Moreover cotyledons, hypocotyls and physiological base cultured on MS medium contained 0.50 mg/l TDZ+ 1.50 mg/l NAA or 1.50 mg/l TDZ+ 0.5 mg/l NAA+ 0.60 mg/l AgNO3 with Gamborg's vitamins and exposed to dark pretreatment for one week at 32±2 ○C gave the highest callus percentage. Data detected three weeks after transferringall cultures to 27±2○C under 16h photoperiod. As for regeneration, physiological base and root which exposed to dark pretreatmentat 32±2 ○C gave the best performance for shoot formation compared to explants cultured at 27±2 ○C under 16 h photoperiod without dark pretreatment. Data clearly indicated that highest percentage of shoot formation was obtained with physiological base cultured on MS medium contained 1.5 mg/l BA with Gamborg's vitamins. While root cultured on MS medium contained 4mg/lBA + 2 mg/l Kin with Gamborg's vitamins significantly increased shoot formation percentage. In that concern, negative results were detected with leaf, cotyledons or hypocotyl cultured on all medium compositions used in this investigation. Keywords : Article : Cite This Article : Hassanien S.H., Ibrahim I.A., Emara H.A., Bekhit M.H., Enass S. Amer, (2013). Effect Of Pretreatment And Media Composition On IN VITRO Propagation Of MOMORDIA CHARANTIA Using Different Explants.. Indian Streams Research Journal, Vol. III, Issue. XI, http://oldisrj.lbp.world/UploadedData/3426.pdf References : - Agarwal S., Shaheen R. 2007. Stimulation of antioxidant system and lipid peroxidation by abiotic stress in leaves of Momordicacharantia. Brazilian J. Plant Physiol., 19:149–161.
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