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Volume : III, Issue : X, November - 2013 Grafting Of Citrus Reticulate Microscions Derived From Nucellar Embryos In Vitro On Volkamariana Rootstock Growing In Greenhouse Ebtsam M. Hamza Ahmed A. Nower Ramadan Aboseree Sayed And Mahdy A. Agwa Published By : Laxmi Book Publication Abstract : Balady mandarin (Citrus. reticulata), belongs to family Rutaceae, is one of the ancestral species of citrus, widely grown in the tropical and subtropical areas. Virus and viroids have been recognized as serious problem limiting the vigor, yield and quality of citrus. These diseases are graft transmissible through infected bud sticks. So, obtained of disease-free plants is imperative to provide certified microscions to the growers and to encourage the planting of grafts instead of seedlings. Micrografting is one of virus-free plant production methods, but, it is complex and its successful percent is low especially it is followed by acclimatization stage. Our paper is the first one to examine producing virus-free microscions via in vitro propagation of vegetative nucellar embryos and examine in vitro treatments which may affect microshoots propagation and successful of grafting the in vitro producing microscions on volkamariana rootstock in greenhouse. Firstly, the sexual nucellar embryo was determined using RAPD marker. Genetic analysis proved that 5th nucellar embryo (the youngest) is differed than the other four embryos. The similarity between them was 84%. Cytokinin types and concentrations affected multiplication of asexual nucellar embryos of Balady mandarin, the highest significant shoot number/explant through second subculture resulted from the explants cultured on MS medium supplemented with 0.75 and 1.00 mg/l BAP (8.80 and 5.60 shoots/explant, respectively). Concerning grafting of in vitro produced microscions in greenhouse, after 45 days of grafting in greenhouse, Balady mandarin scions (microscions) derived from MS medium supplemented with 1.0mg/l Kin or 0.75 mg/l AS maximized successful grafts percentage (37 and 33% successful grafts, respectively). Also, auxin types and concentrations affected successful grafting, microscions derived from MS supplemented with 0.5 mg/l IAA or 0.5 mg/l NAA gave the highest percentage of successful grafts followed by 1.0 mg/l IBA and control (57, 57, 55 and 30% successful grafts, respectively). Microscions derived from MS medium supplemented with 0.6 mg/l PP333 gave the highest percentage of successful grafts 88.77%. Diameter of rootstock and method of grafting also affected grafting successful percent. Producing of microscions in vitro is good tool for producing virus-free plantlet of Balady mandarin. Keywords : Article : Cite This Article : Ebtsam M. Hamza Ahmed A. Nower Ramadan Aboseree Sayed And Mahdy A. Agwa, (2013). Grafting Of Citrus Reticulate Microscions Derived From Nucellar Embryos In Vitro On Volkamariana Rootstock Growing In Greenhouse. Indian Streams Research Journal, Vol. III, Issue. X, http://oldisrj.lbp.world/UploadedData/3317.pdf References : - Ahmad, M., A. Javaid, Hafeez ur-Rahman, S. Hussain, A. Ramzan and Abdul Ghafoor, 2012. Identification of mandarin x orange hybrids Identification of mandarin x orange hybrids using simple sequence repeat markers. J. Agric. Res., 50(2):225-232
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