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Volume : III, Issue : X, November - 2013

PRODUCTION OF HYDROLYTIC ENZYMES BY Beauveria bassiana ISOLATES ON SOLID MEDIUM

Chinnadurai Subramanian And Ganesh Punamalai

Published By : Laxmi Book Publication

Abstract :

The entomopathogenic fungus, Beauveria bassiana is of commercial importance as an alternative to chemical insecticides in an agroecosystem. The fungal pathogen Beauveria bassiana is a widely used mycoinsecticide for control of several insect pests, providing a biological alternative to synthetic chemical insecticides. The potential Beauveria bassiana isolates (Bb – 1, Bb – 2 and Bb – 3) to produce different hydrolytic enzymes such as chitinase, protease, amylase and lipase was determined in the present study by using the specific substrates. Enzyme index value of extracellular enzymes (Chitinase, Protease, Caseinase, Lipase and Amylase) produced by efficient Beauveria bassiana isolates was estimated in the present research. Maximum enzyme activity was observed by the isolate Beauveria bassiana (Bb – 1) followed by Beauveria bassiana (Bb – 2) and Beauveria bassiana (Bb – 3). Chitinase and protease activity of Beauveria bassiana isolates was investigated during Day – 2, Day – 4, Day – 6, Day – 8 and Day – 10. Maximum chitinase and protease activity was observed by the isolate Beauveria bassiana (Bb – 1) at Day – 4 followed by Beauveria bassiana (Bb – 2) and Beauveria bassiana (Bb – 3). Minimum enzyme activity was recorded at Day – 10.

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Chinnadurai Subramanian And Ganesh Punamalai, (2013). PRODUCTION OF HYDROLYTIC ENZYMES BY Beauveria bassiana ISOLATES ON SOLID MEDIUM. Indian Streams Research Journal, Vol. III, Issue. X, http://oldisrj.lbp.world/UploadedData/3292.pdf

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