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Volume : III, Issue : IX, October - 2013 Optimization of acid and enzymatic saccharification of lignocellulosic substrate water hyacinth (EICHHORNIA CRASSIPES) Sirous Kalhorinia , Shaik Naseeruddin , Kothagauni Srilekha Yadav, Jyosthna Khanna Goli And L. Venkateswar Rao Published By : Laxmi Book Publication Abstract : Bioethanol production from lignocelluloses is one of the emerging topics worldwide due to limited sources of fossil fuel and associated environmental problems like global warming and climate change. Water hyacinth (Eichhornia crassipes) with 10% lignin and 65% holocellulose content , is a fast growing aquatic plant exists all over the world. It is a low cost, renewable and a potential source of lignocellulosic feed stock for bioethanol production. In the present work, acid saccharification carried out at optimum condition of temperature (120˚C), acid concentration (2% v/v), biomass loading (1:10 w/v) and time (60 min) yielded 280±11.2 mg/g of total sugars. Further, enzymatic saccharification of remaining substrate under optimum conditions of temperature 50˚C, 150 rpm, biomass loading (1: 5), surfactant concentration (Triton) 0.1% and enzyme accelerase 1500 yielded 340±13.8 mg/g of total sugars. The combined hydrolysis (acid and enzyme) of water hyacinth under optimized conditions resulted in 95.4% of hydrolysis. Keywords : Article : Cite This Article : Sirous Kalhorinia , Shaik Naseeruddin , Kothagauni Srilekha Yadav, Jyosthna Khanna Goli And L. Venkateswar Rao, (2013). Optimization of acid and enzymatic saccharification of lignocellulosic substrate water hyacinth (EICHHORNIA CRASSIPES). Indian Streams Research Journal, Vol. III, Issue. IX, http://oldisrj.lbp.world/UploadedData/3211.pdf References : - Abraham, M., Kurup, G.M., 1996.Bioconversiono of Tapioca (Manihot esculenta) waste and water hyacinth (Eichhornia crassipes ) influence of various physico-chemical factors. J. Ferment. Bioeng. 82, 259-263.
- ]Ashish, K., Singh, L.K., Sanjoy, G., 2009. Bioconversion of lignocellulosic fraction of water-hyacinth (Eichhornia crassipes) hemicellulose acid hydrolysate to ethanol by Pichia stipitis. Bioresour. Technol. 100, 3293–3297.
- Ballesteros, I., Ballesteros, M., Manzanares, P., Negro, M. J., Oliva, J. M.S., Aez, F., 2008. Dilute sulfuric acid pretreatment of cardoon for ethanol production. Biochem. Eng. J. 42, 84–91.
- Burton, J., 2005. Water hyacinth Eichhornia crassipes. Agfact, P7.6.43. P7.6.43, third edition. NSW DPI.
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