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Volume : III, Issue : I, February - 2013 ECOTOXICITY OF HEAVY METAL ON HYDROLYTIC ENZYMES IN FRESHWATER GASTROPOD, BELLAMYA BENGALENSIS FROM HOTGI TANK, SOLAPUR K. R. RAO, P. V. KALE, S. R. ANKARAM, A. R. BIDKAR Published By : Laxmi Book Publication Abstract : The aim of our present investigation is to increase our understanding of the
impact of toxicant on aquatic organism and ecosystem. In the present investigation,
variations in acid and alkaline phosphatase activity of Bellamya bengalensis subjected
to acute toxicity of copper (predetermined 96 hrs LC50 during winter= 2.5 ppm) over a
period of 96 hours during winter season was used to assess ecotoxicity. Samples of
digestive glands, haemolymph, kidney and gills were collected and subjected for
biochemical analysis of enzyme activity at an interval of 24, 48, 72 and 96 hrs after
exposure to 2.5 ppm of copper sulphate. There was generally a gradual increase in the
levels of acid and alkaline phosphatase activity in digestive gland, haemolymph, kidney
and gills when compared to respective control groups. Significant differences were
observed in acid and alkaline phosphatase levels in the copper treated gastropods with
an increase in exposure of time to the copper during winter season. Our results
demonstrate that, a) the activity levels of acid and alkaline phosphatase from different
organs of gastropods exposed to copper depends on type of tissue involved and time of
exposure of metal ions used, b) rapid changes in the activity level of acid and alkaline
phosphatase might be associated with the destabilisation of lysosomal and cell
membranes by metal ions, c) activity levels of hydrolytic enzymes are significantly higher
in digestive glands and haemolymph which indicates that these are the major organ
systems involved in the detoxification of metals Keywords : Article : Cite This Article : K. R. RAO, P. V. KALE, S. R. ANKARAM, A. R. BIDKAR, (2013). ECOTOXICITY OF HEAVY METAL ON HYDROLYTIC ENZYMES IN FRESHWATER GASTROPOD, BELLAMYA BENGALENSIS FROM HOTGI TANK, SOLAPUR. Indian Streams Research Journal, Vol. III, Issue. I, http://oldisrj.lbp.world/UploadedData/2068.pdf References : - Agoes Soegianto, Babang Irawan and Hamami, (2008). Bioaccumulation of Heavy metals in Aquatic animals collected from Coastal Waters of Gresik, Indonesia. Asian Journal of Water, Environment and Pollution, 6(2): 95-100.
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