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Volume : III, Issue : I, February - 2013

ECOTOXICITY OF HEAVY METAL ON HYDROLYTIC ENZYMES IN FRESHWATER GASTROPOD, BELLAMYA BENGALENSIS FROM HOTGI TANK, SOLAPUR

K. R. RAO, P. V. KALE, S. R. ANKARAM, A. R. BIDKAR

Published By : Laxmi Book Publication

Abstract :

The aim of our present investigation is to increase our understanding of the impact of toxicant on aquatic organism and ecosystem. In the present investigation, variations in acid and alkaline phosphatase activity of Bellamya bengalensis subjected to acute toxicity of copper (predetermined 96 hrs LC50 during winter= 2.5 ppm) over a period of 96 hours during winter season was used to assess ecotoxicity. Samples of digestive glands, haemolymph, kidney and gills were collected and subjected for biochemical analysis of enzyme activity at an interval of 24, 48, 72 and 96 hrs after exposure to 2.5 ppm of copper sulphate. There was generally a gradual increase in the levels of acid and alkaline phosphatase activity in digestive gland, haemolymph, kidney and gills when compared to respective control groups. Significant differences were observed in acid and alkaline phosphatase levels in the copper treated gastropods with an increase in exposure of time to the copper during winter season. Our results demonstrate that, a) the activity levels of acid and alkaline phosphatase from different organs of gastropods exposed to copper depends on type of tissue involved and time of exposure of metal ions used, b) rapid changes in the activity level of acid and alkaline phosphatase might be associated with the destabilisation of lysosomal and cell membranes by metal ions, c) activity levels of hydrolytic enzymes are significantly higher in digestive glands and haemolymph which indicates that these are the major organ systems involved in the detoxification of metals

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K. R. RAO, P. V. KALE, S. R. ANKARAM, A. R. BIDKAR, (2013). ECOTOXICITY OF HEAVY METAL ON HYDROLYTIC ENZYMES IN FRESHWATER GASTROPOD, BELLAMYA BENGALENSIS FROM HOTGI TANK, SOLAPUR. Indian Streams Research Journal, Vol. III, Issue. I, http://oldisrj.lbp.world/UploadedData/2068.pdf

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