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Volume : II, Issue : XII, January - 2013 BIOSURFACTANT PRODUCTION USING PINEAPPLE JUICE AS MEDIUM BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE FOREST SOIL. GOVINDAMMAL M AND PARTHASARATHI R Published By : Laxmi Book Publication Abstract : In the present investigation, Pineapple juice has been tested for its suitability
for biosurfactant production using Pseudomonas fluorescens MFS03 isolated from the
crude oil enriched mangrove soil. The strain recorded maximum biosurfactant
production (9.43 g/l) when pineapple juice was used as a medium. Biosurfactant
production was confirmed by conventional screening methods including hemolytic
activity, drop collapsing test, oil displacement method, CTAB, lipase activity and
emulsification index. The isolate shows lowest surface tension of 25.4 mN/m, an
interfacial tension of 0.98 mN/m and a CMC of 34 mg/l. The active compound was
extracted with ethyl acetate. The extract was fractionated by TLC to isolate the pure
compound. Based on the TLC, chromatogram the biosurfactant was identified as
glycolipid type. FT-IR spectrum revealed that the important adsorption bands at
3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1 indicates the chemical structure
of rhamnolipid. The results demonstrated that the Pineapple juice can be a suitable
medium for the biosurfactant production, which can improve the process economical. Keywords : Article : Cite This Article : GOVINDAMMAL M AND PARTHASARATHI R, (2013). BIOSURFACTANT PRODUCTION USING PINEAPPLE JUICE AS MEDIUM BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE FOREST SOIL.. Indian Streams Research Journal, Vol. II, Issue. XII, http://oldisrj.lbp.world/UploadedData/1934.pdf References : - Ashis, K.M and K. Das. 2010. Microbial surfactants and their potential applications. In: Sen, R. (Ed.), Advances in Experimental Medicine and Biology. Biosurfactants, vol. 672. Springer, USA, pp. 54 - 64.
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