|
Volume : II, Issue : XII, January - 2013 BIOSURFACTANT PRODUCTION USING PINEAPPLE JUICE AS MEDIUM BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE FOREST SOIL. GOVINDAMMAL M AND PARTHASARATHI R DOI : 10.9780/22307850, Published By : Laxmi Book Publication Abstract : In the present investigation, Pineapple juice has been tested for its suitability
for biosurfactant production using Pseudomonas fluorescens MFS03 isolated from the
crude oil enriched mangrove soil. The strain recorded maximum biosurfactant
production (9.43 g/l) when pineapple juice was used as a medium. Biosurfactant
production was confirmed by conventional screening methods including hemolytic
activity, drop collapsing test, oil displacement method, CTAB, lipase activity and
emulsification index. The isolate shows lowest surface tension of 25.4 mN/m, an
interfacial tension of 0.98 mN/m and a CMC of 34 mg/l. The active compound was
extracted with ethyl acetate. The extract was fractionated by TLC to isolate the pure
compound. Based on the TLC, chromatogram the biosurfactant was identified as
glycolipid type. FT-IR spectrum revealed that the important adsorption bands at
3466.24, 2926.45, 1743.47, 1407.30 and 1162.26 cm-1 indicates the chemical structure
of rhamnolipid. The results demonstrated that the Pineapple juice can be a suitable
medium for the biosurfactant production, which can improve the process economical. Keywords : Article : Cite This Article : GOVINDAMMAL M AND PARTHASARATHI R, (2013). BIOSURFACTANT PRODUCTION USING PINEAPPLE JUICE AS MEDIUM BY PSEUDOMONAS FLUORESCENS ISOLATED FROM MANGROVE FOREST SOIL.. Indian Streams Research Journal, Vol. II, Issue. XII, DOI : 10.9780/22307850, http://oldisrj.lbp.world/UploadedData/1934.pdf References : - Ashis, K.M and K. Das. 2010. Microbial surfactants and their potential applications. In: Sen, R. (Ed.), Advances in Experimental Medicine and Biology. Biosurfactants, vol. 672. Springer, USA, pp. 54 - 64.
- Banat, I.M., R.S. Makkar, S.S. Cameotra. 2000. Potential commercial applications of microbial surfactants. Appl and Environ Microbiol., 53: 495 - 508.
- Benincasa, M., M. arques, A . Pinazo, A. Manresa. 2010. Rhamnolipid surfactants: alternative substrates,new strategies. In: Sen, R. (Ed.), Advances in Experimental Medicine and Biology. Biosurfactants, vol.672. Springer, USA,pp. 170-184.
- Bodour, A.A., and R.M. Miller-Maier. 1998. Application of a Modified drop collapsing Technique for Surfactant Quantitation and Screening of Biosurfactant-producing Microorganisms. J. Microbiol. Methods., 32: 273-280.
- Buchanan, R.E., N.E. Gibbons, S.T. Cowan, T.G. Holt, J. Liston, R.G. Murry, C.F. Niven, A.W. Ravin, R.Y. Stainer. 1974. Bergey's manual of determinative bacteriology. Williams and Wilkins Co.,Baltimore,1246 p.
- Cameotra, S.S., and R.S. Makkar. 1998. Synthesis of biosurfactants in extreme conditions. Applied Microbiol and Biotechnol 50: 520 - 529.
- Cappuccino, J.G., Sherman, N., 1999. Microbiology: a laboratory manual. Pearson Education, Singapore,pp. 49.
- Carillo, P., Mardarz, C., Pitta, Alvarez, S., 1996. Isolation and selection of biosurfactant production bacteria. World. J. Microbiol. Biotechnol. 12: 82-84.
- Carrera, P., Cosmina, P., Grandi, G., Guido., 1993. Method of producing surfactin with the use of mutant of Bacillus. United State Patent: 5227294.
- Chaplin, M.F and J.F. Kennedy. 1994. In Carbohydrate Analysis. A Practical Approach. 2nd ed., IRL Press,ISBN: 0947946446.
- Cooper, D.G and B.G. Goldenberg. 1987. Surface-active agents from two Bacillus species. Appl. Environ. Microbiol., 53: 224-229.
- Cooper, D.G., MacDonald, C.R., Duff, S.J.B., Kosaric, N., 1981. Enhanced production of surfactin from B. subtilisby continuous product removal and metal cation additions. Appl. Environ.Microbiol. 42: 408–412.
- Desai, J.D. and I.M. Banat, 1997. Microbial production of biosurfactants and their commercial potential. Mcrobiol. Mol. Biol. Rev. 61: 41- 64.
|
Article Post Production
Article Indexed In
|