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Volume : II, Issue : IX, October - 2012 Isolation Of Bacteria From Personal Computer Hardware And Sequence Analysis Of Hypervariable Region V6 For Molecular Identification V. S. Wadhai and Varsha N. Tandra Published By : Laxmi Book Publication Abstract : The usage of computers is increasing day by day. Computers just like microbes
are ubiquitous and they continue to have an increased presence in almost every aspect of
our occupational, recreational, and residential environment. This research seeks to
investigate what kinds of microorganisms, especially bacteria that actually can
contaminate computer hardware. Bacterial 16S ribosomal RNA genes generally contain
nine “hypervariable regions” (V1 – V9) that demonstrate considerable sequence
diversity among different bacterial species and can be used for species identification. We
identified the unknown bacteria isolated from computer hardware by molecular
detection and sequence analysis of 16S ribosomal RNA hypervariable region V6.The
gene sequence obtained was studied in the region from V3-V6. Sequence similarity
dendogram were created for hypervariable regions V3-V6. After complete analysis and
using bioinformatics tools our investigation demonstrated that the V6 (nucleotide 986-
1043) contains diverse character & maximum distinguish power for the Bacillus subtilis
species analyzed. Keywords : Article : Cite This Article : V. S. Wadhai and Varsha N. Tandra, (2012). Isolation Of Bacteria From Personal Computer Hardware And Sequence Analysis Of Hypervariable Region V6 For Molecular Identification. Indian Streams Research Journal, Vol. II, Issue. IX, http://oldisrj.lbp.world/UploadedData/1410.pdf References : - Baker G.C., Smith, J.J. and Cowan D.A., Review and re-analysis of domain-specific 16S primers, J. Microbiol. Methods 55(3):541-551(2003)
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