Indian Streams Research Journal

Volume : VI, Issue : IV, May - 2016

SCREENING AND OPTIMIZATION OF POLYHYDROXYBUTYRATE (PHB)FROM AZOTOBACTER SPP

D. Lakshmi priya, Ramya Anandan and P. Rajendran

By : Laxmi Book Publication

Abstract :

Azotobacter spp is a obligate aerobe that is able to fix nitrogen and has a remarkable machinery to synthesis an intra cellular polyester Poly B hydroxybutryate.This experiment was done to qualitatively and quantitatively screen the soil isolates for PHB production. Study on the growth and production of PHB was done at standard pH and temperature.

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References :

1. Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
2. Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
3. Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
4. Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
5. Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
6. Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
7. Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
8. Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
9. Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
10. Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
11. Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
12. Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
13. Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
14. Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
15. Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
16. Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
17. Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
18. Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
19. Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
20. Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
21. Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
22. Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
23. Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
24. Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
25. Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
26. Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
27. Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
28. Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
29. Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
30. Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.

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