Volume : VI, Issue : IV, May - 2016 SCREENING AND OPTIMIZATION OF POLYHYDROXYBUTYRATE (PHB)FROM AZOTOBACTER SPP ![](ArticleImage/8189.jpg) D. Lakshmi priya, Ramya Anandan and P. Rajendran By : Laxmi Book Publication Abstract : Azotobacter spp is a obligate aerobe that is able to fix nitrogen and has a remarkable machinery to synthesis an intra cellular polyester Poly B hydroxybutryate.This experiment was done to qualitatively and quantitatively screen the soil isolates for PHB production. Study on the growth and production of PHB was done at standard pH and temperature. Keywords : Article : Cite This Article : D. Lakshmi priya, Ramya Anandan and P. Rajendran (2016). SCREENING AND OPTIMIZATION OF POLYHYDROXYBUTYRATE (PHB)FROM AZOTOBACTER SPP . Indian Streams Research Journal, Vol. VI, Issue. IV, http://isrj.org/UploadedData/8189.pdf References : - Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
- Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
- Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
- Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
- Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
- Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
- Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
- Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
- Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
- Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
- Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
- Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
- Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
- Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
- Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
- Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
- Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
- Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
- Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
- Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
- Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
- Steinbüchel, A. and Valentin, H.E.,( 1995). Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiology Letters,Vol.128 No.3, pp.219-228.
- Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
- Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
- Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
- Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
- Byrom, D., 1987. Polymer synthesis by microorganisms: technology and economics. Trends in Biotechnology, Vol.5 No.9, pp.246-250.
- Doi, Y., Segawa, A. and Kunioka, M., (1990). Biosynthesis and characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. International Journal of Biological Macromolecules,Vol.12 No.2, pp.106-111.
- Segura, D., Guzmán, J. and Espín, G., (2003). Azotobacter vinelandii mutants that overproduce poly-β-hydroxybutyrate or alginate. Applied microbiology and biotechnology, Vol.63 No.2, pp.159-163.
- Gouda, M.K., Swellam, A.E. and Omar, S.H., (2001). Production of PHB by a Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological research,Vol.156 No.3, pp.201-207.
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