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Volume : III, Issue : XII, January - 2014

AN EFFICIENT IN VITRO REGENERATION OF SHOOT FROM COTYLEDON DERIVED CALLUS OF PIGEON PEA (CAJANUS CAJAN (L.) MILLSP.)

M. Prabhakaran, S. Elumalai

By : Laxmi Book Publication

Abstract :

Pigeon pea (Cajanus cajan (L.) Millsp.) is an important grain legume of the semi-arid tropics. It provides protein rich food. Pigeon pea being recalcitrant species, regeneration of multiple shoots via callus induction and organogenesis was achieved from cotyledon explants of pigeon pea. Callus induction and shoot regeneration at various frequencies were observed using different concentration and combination of growth regulators. The highest callus formation was observed on MS medium + 1.0 m-1 g l IAA + 0.9 mg -l1 Kinetin. The Highest shoot formation was obtained on MS medium fortified with 2.0 mg l-1 BAP + 0.2 mg l-1 NAA and 0.4 mg -l1 GA. Regenerated plants were successfully established in the soil 3 after acclimatization.

Keywords :


Article :


Cite This Article :

M. Prabhakaran, S. Elumalai(2014). AN EFFICIENT IN VITRO REGENERATION OF SHOOT FROM COTYLEDON DERIVED CALLUS OF PIGEON PEA (CAJANUS CAJAN (L.) MILLSP.). Indian Streams Research Journal, Vol. III, Issue. XII, http://isrj.org/UploadedData/4098.pdf

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  167. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
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  170. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
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  176. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  177. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  178. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
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  181. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  182. Ebida A I A & Hu C, In vitro morphogenic responses and plant regeneration from pepper (Capsicum annum L.cv Early
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  186. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  187. Ebida A I A & Hu C, In vitro morphogenic responses and plant regeneration from pepper (Capsicum annum L.cv Early
  188. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
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  190. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  191. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  192. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  193. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  194. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  195. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  196. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  197. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  198. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  199. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  200. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  201. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  202. Tissue Organ Cult, 53 (1998) 217-220.
  203. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  204. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  205. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  206. Lac Production, 2 (1978) 1-4.
  207. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  208. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  209. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  210. Tissue Organ Cult, 53 (1998) 217-220.
  211. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  212. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  213. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  214. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  215. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  216. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  217. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  218. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  219. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  220. Ebida A I A & Hu C, In vitro morphogenic responses and plant regeneration from pepper (Capsicum annum L.cv Early
  221. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  222. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  223. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  224. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  225. Tissue Organ Cult, 53 (1998) 217-220.
  226. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  227. Tissue Organ Cult, 53 (1998) 217-220.
  228. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  229. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  230. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  231. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  232. Ebida A I A & Hu C, In vitro morphogenic responses and plant regeneration from pepper (Capsicum annum L.cv Early
  233. Tissue Organ Cult, 53 (1998) 217-220.
  234. Tissue Organ Cult, 53 (1998) 217-220.
  235. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  236. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  237. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  238. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  239. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  240. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  241. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  242. Lac Production, 2 (1978) 1-4.
  243. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  244. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  245. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  246. Lac Production, 2 (1978) 1-4.
  247. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  248. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  249. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  250. Lac Production, 2 (1978) 1-4.
  251. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  252. Ebida A I A & Hu C, In vitro morphogenic responses and plant regeneration from pepper (Capsicum annum L.cv Early
  253. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  254. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  255. Tissue Organ Cult, 53 (1998) 217-220.
  256. Lac Production, 2 (1978) 1-4.
  257. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  258. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  259. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  260. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  261. Ebida A I A & Hu C, In vitro morphogenic responses and plant regeneration from pepper (Capsicum annum L.cv Early
  262. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  263. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  264. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  265. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  266. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  267. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  268. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  269. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  270. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  271. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  272. Tissue Organ Cult, 53 (1998) 217-220.
  273. Tissue Organ Cult, 53 (1998) 217-220.
  274. Lac Production, 2 (1978) 1-4.
  275. acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult, 35 (1993) 223-227.
  276. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  277. Tissue Organ Cult, 53 (1998) 217-220.
  278. Tissue Organ Cult, 53 (1998) 217-220.
  279. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  280. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  281. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  282. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  283. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  284. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  285. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  286. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  287. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  288. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  289. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  290. Eapen S & George L, Plant regeneration from leaf discs of peanut and pigeon pea: influence of benzyladenine, indoleacetic
  291. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  292. Tissue Organ Cult, 53 (1998) 217-220.
  293. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  294. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  295. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  296. Ebida A I A & Hu C, In vitro morphogenic responses and plant regeneration from pepper (Capsicum annum L.cv Early
  297. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  298. pigeon pea (Cajanus cajan [L.] Millsp.) using leaf explants, Plant Cell Rep, 21 (2003)1072-1079.
  299. .Anonymous, A summary of high-yield lac production. Research group for high-yield lac production, Developing Status of
  300. Dayal S, Lavanya M, Devi P & Sharma K K, An efficient protocol for shoot regeneration and genetic transformation of
  301. Lac Production, 2 (1978) 1-4.
  302. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  303. Eapen S, Tivarekar S & George L, Thidiazuron-induced shoot regeneration in pigeon pea (Cajanus cajan L.), Plant Cell
  304. Lac Production, 2 (1978) 1-4.
  305. Frankalin G, Jayachandran R, Melehias G & Ignacimuthu S, Multiple shoot induction and regeneration of pigeon pea
  306. (Cajanus cajan (L.) Millsp.) cv. Vamban 1 from apical and axillary meristem, Curr. Sci, 74 (1998) 936-937.
  307. California Wonder) seedling explants, Plant Cell Rep, 13 (1993) 107-110.
  308. Tissue Organ Cult, 53 (1998) 217-220.
  309. Tissue Organ Cult, 53 (1998) 217-220.

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