Indian Streams Research Journal

Volume : IV, Issue : I, February - 2014

MICROBIAL PROTEASE: A POTENT INDUSTRIAL TOOL

Pallavi Aradhye, Meera Chavan

By : Laxmi Book Publication

Abstract :

Microbial proteases are among the most important hydrolytic enzymes and have been studies extensively since the advent of enzymology. Proteases not only play important role in cellular metabolic activity but also gained considerable attention in the industrial community. Bacillus Stearothermophilus is used for the production of enzyme. It was carried out by using soy meal and wheat husk protein source at 330C ± 20C temperature with 7.2 pH for 5 days of incubation. The crude enzyme was then checked against different proteinaceous substances, such as blood clot, blood stain feathers, x-ray film, milk coagulation, casein hydrolysis etc. All these substrates get acted upon by proteases enzyme at 370C of temperature within 19 to 94 hrs of incubation. Enzyme shows, 0.478 mg protein/ml, 226.2U/ml activity and 556.9 U/mg protein in the extracted broth.

Keywords :

• blood clot lysis
• stain removal
• detergent
• Microbial proteases
• proteolytic
• caseinolytic assay
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent
• stain removal
• detergent
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis

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References :

1. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
2. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
3. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
4. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
5. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
6. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
7. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
8. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
9. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
10. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
11. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
12. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
13. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
14. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
15. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
16. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
17. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
18. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
19. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
20. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
21. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
22. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
23. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
24. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
25. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
26. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
27. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
28. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
29. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
30. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
31. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
32. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
33. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
34. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
35. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
36. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
37. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
38. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
39. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
40. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
41. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
42. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
43. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
44. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
45. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
46. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
47. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
48. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
49. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
50. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
51. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
52. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
53. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
54. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
55. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
56. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
57. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
58. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
59. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
60. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
61. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
62. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
63. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
64. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
65. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
66. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
67. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
68. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
69. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
70. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
71. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
72. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
73. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
74. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
75. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
76. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
77. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
78. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
79. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
80. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
81. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
82. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
83. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
84. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
85. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
86. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
87. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
88. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
89. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
90. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
91. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
92. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
93. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
94. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
95. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
96. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
97. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
98. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
99. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
100. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
101. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
102. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
103. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
104. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
105. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
106. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
107. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
108. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
109. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
110. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
111. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
112. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
113. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
114. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
115. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
116. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
117. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
118. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
119. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
120. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
121. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
122. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
123. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
124. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
125. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
126. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
127. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
128. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
129. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
130. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
131. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
132. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
133. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
134. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
135. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
136. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
137. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
138. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
139. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
140. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
141. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
142. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
143. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
144. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
145. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
146. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
147. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
148. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
149. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
150. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
151. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
152. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
153. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
154. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
155. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
156. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
157. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
158. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
159. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
160. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
161. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
162. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
163. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
164. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
165. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
166. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
167. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
168. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
169. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
170. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
171. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
172. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
173. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
174. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
175. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
176. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
177. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
178. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
179. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
180. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
181. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
182. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
183. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
184. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
185. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
186. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
187. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
188. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
189. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
190. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
191. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl Microbiol Biotechnol 59:15–32.
192. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.
193. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
194. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62:597–635.
195. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
196. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
197. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
198. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
199. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
200. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
201. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
202. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627.

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