|
Volume : III, Issue : I, February - 2013 ECOTOXICITY OF HEAVY METAL ON HYDROLYTIC ENZYMES IN FRESHWATER GASTROPOD, BELLAMYA BENGALENSIS FROM HOTGI TANK, SOLAPUR K. R. RAO, P. V. KALE, S. R. ANKARAM, A. R. BIDKAR DOI : 10.9780/22307850, Published By : Laxmi Book Publication Abstract : The aim of our present investigation is to increase our understanding of the
impact of toxicant on aquatic organism and ecosystem. In the present investigation,
variations in acid and alkaline phosphatase activity of Bellamya bengalensis subjected
to acute toxicity of copper (predetermined 96 hrs LC50 during winter= 2.5 ppm) over a
period of 96 hours during winter season was used to assess ecotoxicity. Samples of
digestive glands, haemolymph, kidney and gills were collected and subjected for
biochemical analysis of enzyme activity at an interval of 24, 48, 72 and 96 hrs after
exposure to 2.5 ppm of copper sulphate. There was generally a gradual increase in the
levels of acid and alkaline phosphatase activity in digestive gland, haemolymph, kidney
and gills when compared to respective control groups. Significant differences were
observed in acid and alkaline phosphatase levels in the copper treated gastropods with
an increase in exposure of time to the copper during winter season. Our results
demonstrate that, a) the activity levels of acid and alkaline phosphatase from different
organs of gastropods exposed to copper depends on type of tissue involved and time of
exposure of metal ions used, b) rapid changes in the activity level of acid and alkaline
phosphatase might be associated with the destabilisation of lysosomal and cell
membranes by metal ions, c) activity levels of hydrolytic enzymes are significantly higher
in digestive glands and haemolymph which indicates that these are the major organ
systems involved in the detoxification of metals Keywords : Article : Cite This Article : K. R. RAO, P. V. KALE, S. R. ANKARAM, A. R. BIDKAR, (2013). ECOTOXICITY OF HEAVY METAL ON HYDROLYTIC ENZYMES IN FRESHWATER GASTROPOD, BELLAMYA BENGALENSIS FROM HOTGI TANK, SOLAPUR. Indian Streams Research Journal, Vol. III, Issue. I, DOI : 10.9780/22307850, http://oldisrj.lbp.world/UploadedData/2068.pdf References : - Agoes Soegianto, Babang Irawan and Hamami, (2008). Bioaccumulation of Heavy metals in Aquatic animals collected from Coastal Waters of Gresik, Indonesia. Asian Journal of Water, Environment and Pollution, 6(2): 95-100.
- Anon, (1963). The colorimetric determination of phosphatase. Sigma Tech. Bull.104: Sigma Chemicals Co. St. Louis.
- Atli, G. and Canli, M. (2007). Enzymatic responses to metal exposures in a freshwater fish Oreochromis niloticus. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 145(2): 282- 287.
- Bailey, N. T. J. (1965). Statistical methods in biology. ELBS, English University press, London
- Cheng, T. C., (1983). Triggering of immunologic defense mechanisms of molluscan shellfish by biotic and abiotic challenge and its application. Mar. Technol. Soc. J., 17:18-25.
- Finney, D. J. (1971). Probit Analysis (3rd Edition). Cambridge University Press.
- Jayakumar, P., Jothivel, N. and Paul, V. I. (2008). Heavy metals induced alterations in the acid phosphatase activity in the edible freshwater mussel Lamellidens marginalis (Lamarck). The Internet Journal of Toxicology, 5(2).
- Karan, V., Vitorovic, S., Tutundzic, V. and Poleksic, V., (1998). Functional enzymes activity and gill histology of carp after copper sulfate exposure and recovery. Ecotoxicology and Environmental Safety, 40(1-2): 49-55.
- Lodhi, H. S., Khan, M. A., Verma, R. S. and Sharma, U. D. (2006). Acute toxicity of copper sulphate to fresh water prawns. Journal of Environmental Biology, 27(3): 585-588.
- Mason, C. F., (1996). Biology of fresh water pollution (IIIrdEd.). Department of Biology, university of Essex. Paletisher – Longman, Singapore, Paletishers (1996).
- Nurnberg, H. W., (1984). Bio-accumulation of heavy metals by bivalves from Lim Fjord (North Adriatic Sea). Journal of Marine Biological Sciences 81(2): 177-188.
- Rahman, M. F. and Siddiqui, M. K. J., (2004). Biochemical effects of vepacide (from Azadurachta indica) on Wistar rats during subchronic exposure. Ecotoxicology and Environmental Safety, 59(3): 332- 339.
- Rajakajshmi, S. and Mohandas, A., (2005). Copper- induced changes in tissue enzyme activity in fresh water mussel. Ecotoxicology and Environmental Safety, 62(1): 140-143.
- Ram, R. N. and Sathyanesen, A. G. (1985). Mercuruc chloride, cythion and ammonium sulfate induced changes in the brain, liver and ovarian alkaline phosphatase content in the fish Channa puntactus. Environ. Ecol., 3: 263-268.
- Regoli, F. and Principato, G., (1995). Glutathione, glutathione–dependent and antioxidant enzymes in mussel Mytilus galloprovincialis, exposed to metals under field and laboratory conditions: implications for the use of biochemical biomarkers. Aquatic toxicology, 31(2): 143-164.
- S. R. Krupesha Sharma, P. K. Asokan and A. K Sahoo, (2006). Effect of sub-lethal level of copper and mercury on digestive gland cells and lysosomal enzyme activity in the green mussel (Perna viridis L). J. Mar. Ass. India, 48(2): 245-248.
|
Article Post Production
Article Indexed In
|