Indian Streams Research Journal

Volume : IV, Issue : I, February - 2014

MICROBIAL PROTEASE: A POTENT INDUSTRIAL TOOL

Pallavi Aradhye, Meera Chavan

DOI : 10.9780/22307850, By : Laxmi Book Publication

Abstract :

Microbial proteases are among the most important hydrolytic enzymes and have been studies extensively since the advent of enzymology. Proteases not only play important role in cellular metabolic activity but also gained considerable attention in the industrial community. Bacillus Stearothermophilus is used for the production of enzyme. It was carried out by using soy meal and wheat husk protein source at 303C ±0 2C temperature with 7.2 pH for 5 days of incubation. The crude enzyme was then checked against different proteinaceous substances, such as blood clot, blood stain feathers, x-ray film, milk coagulation, casein hydrolysis etc. All these substrates get acted upon by proteases enzyme at 370C of temperature within 19 to 94 hrs of incubation. Enzyme shows, 0.478 mg protein/ml, 226.2U/ml activity and 556.9 U/mg protein in the extracted broth.

Keywords :

• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• Microbial proteases
• proteolytic
• caseinolytic assay
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• blood clot lysis
• stain removal
• detergent.
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• Microbial proteases
• proteolytic
• caseinolytic assay
• blood clot lysis
• stain removal
• detergent.
• stain removal
• detergent.

Article :

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References :

1. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
2. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
3. Microbiol Biotechnol 59:15–32.
4. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
5. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
6. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
7. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
8. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
9. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
10. Microbiol Biotechnol 59:15–32.
11. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
12. Microbiol Biotechnol 59:15–32.
13. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
14. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
15. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
16. Microbiol Biotechnol 59:15–32.
17. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
18. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
19. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
20. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
21. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
22. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
23. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
24. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
25. Microbiol Biotechnol 59:15–32.
26. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
27. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
28. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
29. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
30. Microbiol Biotechnol 59:15–32.
31. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
32. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
33. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
34. Microbiol Biotechnol 59:15–32.
35. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
36. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
37. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
38. Microbiol Mol Biol Rev 62:597–635.
39. Microbiol Biotechnol 59:15–32.
40. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
41. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
42. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
43. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
44. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
45. Microbiol Biotechnol 59:15–32.
46. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
47. Microbiol Mol Biol Rev 62:597–635.
48. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
49. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
50. Microbiol Biotechnol 59:15–32.
51. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
52. Microbiol Mol Biol Rev 62:597–635.
53. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
54. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
55. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
56. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
57. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
58. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
59. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
60. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
61. Microbiol Biotechnol 59:15–32.
62. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
63. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
64. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
65. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
66. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
67. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
68. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
69. Microbiol Mol Biol Rev 62:597–635.
70. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
71. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
72. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
73. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
74. Microbiol Mol Biol Rev 62:597–635.
75. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
76. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
77. Microbiol Biotechnol 59:15–32.
78. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
79. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
80. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
81. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
82. Microbiol Mol Biol Rev 62:597–635.
83. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
84. Microbiol Biotechnol 59:15–32.
85. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
86. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
87. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
88. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
89. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
90. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
91. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
92. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
93. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
94. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
95. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
96. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
97. Microbiol Mol Biol Rev 62:597–635.
98. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
99. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
100. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
101. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
102. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
103. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
104. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
105. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
106. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
107. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
108. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
109. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
110. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
111. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
112. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
113. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
114. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
115. Microbiol Biotechnol 59:15–32.
116. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
117. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
118. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
119. Microbiol Mol Biol Rev 62:597–635.
120. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
121. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
122. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
123. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
124. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
125. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
126. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
127. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
128. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
129. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
130. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
131. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
132. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
133. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
134. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
135. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
136. Microbiol Biotechnol 59:15–32.
137. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
138. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
139. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
140. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
141. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
142. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
143. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
144. Microbiol Mol Biol Rev 62:597–635.
145. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
146. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
147. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
148. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
149. Microbiol Mol Biol Rev 62:597–635.
150. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
151. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
152. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
153. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
154. Microbiol Mol Biol Rev 62:597–635.
155. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
156. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
157. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
158. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
159. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
160. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
161. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
162. Microbiol Mol Biol Rev 62:597–635.
163. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
164. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
165. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
166. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
167. Microbiol Biotechnol 59:15–32.
168. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
169. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
170. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
171. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
172. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
173. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
174. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
175. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
176. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
177. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
178. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
179. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
180. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
181. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
182. Microbiol Biotechnol 59:15–32.
183. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
184. Microbiol Biotechnol 59:15–32.
185. Microbiol Biotechnol 59:15–32.
186. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
187. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
188. Microbiol Biotechnol 59:15–32.
189. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
190. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
191. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
192. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
193. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
194. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
195. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
196. Microbiol Mol Biol Rev 62:597–635.
197. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
198. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
199. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
200. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
201. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
202. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
203. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
204. Microbiol Mol Biol Rev 62:597–635.
205. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
206. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
207. Microbiol Biotechnol 59:15–32.
208. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
209. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
210. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
211. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
212. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
213. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
214. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
215. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
216. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
217. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
218. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
219. Microbiol Mol Biol Rev 62:597–635.
220. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
221. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
222. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
223. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
224. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
225. Microbiol Biotechnol 59:15–32.
226. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
227. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
228. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
229. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
230. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
231. Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases.
232. Microbiol Biotechnol 59:15–32.
233. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
234. Alagarsamy Sumantha1,2, Christian Larroche2 and Ashok Pandey (2006) Microbiology and Industrial Biotechnology of
235. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
236. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
237. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
238. Microbiol Mol Biol Rev 62:597–635.
239. Abhijit Ray (2012) Protease Enzyme- Potential Industrial ScopeInt. J. Tech. Vol. 2: Issue 1, 01-04.
240. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
241. Food-Grade Proteases: A Perspective Food Technol. Biotechnol. 44 (2) 211–220.
242. Feroz Khan (2013), New microbial proteases in leather and detergent industries.Innov. Res. Chem. 1:1 1-6.
243. Folin O. and Ciocalteau V.(1929) Journal of Biology and Chemistry, 73, 627
244. Microbiol Mol Biol Rev 62:597–635.
245. R. Gupta, Q.K. Beg, P. Lorenz (2002) Bacterial alkaline proteases: molecular approaches and industrial applications Appl
246. Kalisz HM (1988) Microbial proteinases. Adv Biochem Eng Biotechnol 36:1–65.
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