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Volume : III, Issue : III, April - 2013 ISOLATION AND EXTRACTION OF BACTERIOCINS PRODUCED BY LACTIC ACID BACTERIA ISOLATED FROM RAW MILK SAMPLES DESHMUKH P.V. AND THORAT P.R. DOI : 10.9780/22307850, Published By : Laxmi Book Publication Abstract : Isolation and screening of microorganisms from naturally occurring processes
have always been the most powerful means for obtaining useful cultures for scientific
and commercial purposes.Lactic acid bacteria commonly used as a natural food
preservative, which produces certain antimicrobial substances such as Bacteriocin. The
present study deals with isolation and characterization of Bacteriocin producing
Lactobacillus sp., from a raw unpasteurized milk sample. Screening and isolation was
done on MRS agar, and isolates were identified using biochemical methods. Bacteriocin
was produced from promising isolate. Bacteriocin from fermented broth was extracted
by solvent extraction and antibacterial activity was determined against selected test
microorganisms of both Gram positive and Gram negative groups.This study revealed
the possibility of using Bacteriocin as food biopreservative to control food spoilage and
pathogenic bacteria. Keywords : Article : Cite This Article : DESHMUKH P.V. AND THORAT P.R., (2013). ISOLATION AND EXTRACTION OF BACTERIOCINS PRODUCED BY LACTIC ACID BACTERIA ISOLATED FROM RAW MILK SAMPLES. Indian Streams Research Journal, Vol. III, Issue. III, DOI : 10.9780/22307850, http://oldisrj.lbp.world/UploadedData/2268.pdf References : - 1.Arokiyamary, A., and Shivkumar, P.K., (2011), Antibacterial activity of bacteriocin producing Lactobacillus sp., isolated from traditional milk products. Current Botany, 2(3):5 – 8.
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